%0 Journal Article
%T Preparation and bioactivity of anti-human red blood cell ScFv and CSFV E2 bifunctional fusion protein<br>抗人红细胞单链抗体与猪瘟E2蛋白双功能融合蛋白的构建及生物学活性检测
%A Shaomin Qin
%A Anbin Bai
%A Jianmin Wu
%A Wenjun Liao
%A Shuzhi Yuan
%A Jun Hua
%A Zhongyi Guan
%A <br>覃绍敏
%A 白安斌
%A 吴健敏
%A 廖文军
%A 袁书智
%A 华俊
%A 关忠谊
%J 生物工程学报
%D 2010
%I 
%X The aim of this study is to construct a bifunctional fusion protein, which can conjugate both human red blood cells and antibodies against classical swine fever virus (CSFV). We respectively amplified 2E8ScFv and mE2 genes from different recombinant vectors, in which 2E8ScFv gene is the single chain Fv gene against H antigen of human red blood cells, whereas mE2 gene is the main antigen coding region gene of CSFV E2 protein. We used overlap extension PCR to obtain an artificial fusion gene segment 2E8mE2 co...
%K classical swine fever virus (CSFV)
%K ScFv
%K bifunctional protein
%K prokaryotic expression
%K erythrocyte agglutination test<br>猪瘟病毒
%K 单链抗体
%K 双功能蛋白
%K 原核表达
%K 红细胞凝集试验
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=4E7A018FD92D7E524DC1043B775C4F09&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=CA4FD0336C81A37A&sid=D3E34374A0D77D7F&eid=339D79302DF62549&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0