Construction of Novel Recombinant Strain Harboring Glycerol Dehydratase Reactivating Factor Capable of Producing 1,3-propanediol
含甘油脱水酶激活因子编码基因的产1,3-丙二醇新型重组菌的构建

The dhaB gene encoding glycerol dehydratase and dhaG dhaF gene encoding glycerol dehydratase reactivating factor from Citrobacter freundii were amplified by PCR.The temperature control expression vector pHsh harboring yqhD,dhaB,dhaG and dhaF gene was transformed into E.coli JM109 to yield the recombinant strain E.coli JM109(pHsh-dhaB-dhaG-dhaF-yqhD).The results from SDS-PAGE analysis show that the recombinant product was consistent with the molecular weight predicted from gene sequence.The fermentation result show that the yield of 1,3-propanediol was increased by 28% compared with E.coli JM109(pHsh-dhaB-yqhD).