%0 Journal Article
%T Construction of Novel Recombinant Strain Harboring Glycerol Dehydratase Reactivating Factor Capable of Producing 1,3-propanediol<br>含甘油脱水酶激活因子编码基因的产1,3-丙二醇新型重组菌的构建
%A ZHANG Xiao-Mei
%A ZHUGE Jian
%A <br>张晓梅
%A 诸葛健
%J 生物工程学报
%D 2007
%I 
%X The dhaB gene encoding glycerol dehydratase and dhaG dhaF gene encoding glycerol dehydratase reactivating factor from Citrobacter freundii were amplified by PCR.The temperature control expression vector pHsh harboring yqhD,dhaB,dhaG and dhaF gene was transformed into E.coli JM109 to yield the recombinant strain E.coli JM109(pHsh-dhaB-dhaG-dhaF-yqhD).The results from SDS-PAGE analysis show that the recombinant product was consistent with the molecular weight predicted from gene sequence.The fermentation result show that the yield of 1,3-propanediol was increased by 28% compared with E.coli JM109(pHsh-dhaB-yqhD).
%K 1<br>甘油脱水酶激活因子
%K 重组大肠杆菌
%K 3-丙二醇
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=4B8366EF4D8EA657&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=94C357A881DFC066&sid=2EA35D7E5C9A6E66&eid=450524CAAD043961&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=16