Research of the Feeding Strategy in the Fermentation of Recombinant Human Fibreblast Growth Factor Mutant
重组人酸性成纤维细胞生长因子改构体发酵的补料-分批策略研究

Human acidic fibreblast growth factor(haFGF) was a kind of cell growth factor with wide bio-activity on cell from mesectoderm and neuro-ectoderm.In this paper, the effect of acetate concentration on the growth and expression of recombinant human acidic fibroblast growth factor mutant system E.coli BL21(DE3)/pET3C-haFGF was investigated. Four fed-batch modes: batch-fed, batch-DO static balance, DO static balance-glucose starvation, and pH-static state were investigated. The accumulation of acetate during the fermentation course was effectively inhibited. The OD- 600nm value was about 22,after purification, the soluble rhaFGF yielded 450mg/L.During the fermentation, no special ways such as pure oxygen, pressure were adopted, thus the established process would be easily scaled up for industry purpose.