%0 Journal Article
%T Research of the Feeding Strategy in the Fermentation of Recombinant Human Fibreblast Growth Factor Mutant<br>重组人酸性成纤维细胞生长因子改构体发酵的补料-分批策略研究
%A YUAN Hui
%A LI Xiao-Kun
%A YANG Shu-Lin
%A <br>袁辉
%A 李校堃
%A 杨树林
%J 生物工程学报
%D 2006
%I 
%X Human acidic fibreblast growth factor(haFGF) was a kind of cell growth factor with wide bio-activity on cell from mesectoderm and neuro-ectoderm.In this paper, the effect of acetate concentration on the growth and expression of recombinant human acidic fibroblast growth factor mutant system E.coli BL21(DE3)/pET3C-haFGF was investigated. Four fed-batch modes: batch-fed, batch-DO static balance, DO static balance-glucose starvation, and pH-static state were investigated. The accumulation of acetate during the fermentation course was effectively inhibited. The OD- 600nm value was about 22,after purification, the soluble rhaFGF yielded 450mg/L.During the fermentation, no special ways such as pure oxygen, pressure were adopted, thus the established process would be easily scaled up for industry purpose.
%K recombinant human fibreblast growth factor
%K Escherichia coli
%K fed-batch
%K DO static balance
%K glucose starvation
%K pH static state<br>重组人酸性成纤维细胞生长因子
%K 大肠杆菌
%K 流加-分批
%K 静态DO平衡
%K 葡萄糖饥饿法
%K 静态pH
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=250E56A1674FB17C&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=0B39A22176CE99FB&sid=3224764AEAFCF8C2&eid=170CE8B011EA4FD9&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=16