The Mechanism of Stat3 Nuclear Import
STAT3 入核分子机制的初步研究

In order to investigate the mechanism of stat3 nuclear import. positio ned a characterized NLS of the SV40 large T antigen into Stat3\|GFP ,Dstat3\|GF P respectively between the C\|terminus of Stat3 and the N\|terminus of GFP to cr eate Stat3\|NLS\|GFP and Dstat3\|NLS\|GFP. With NLS\|GFP as the positive contro l, Expression of the Stat3\|NLS\|GFP without IL\|6 stimulation and Stat3\|GFP wi th IL\|6 stimulation resulted in a predominantly nuclear localization in 293T ce ll. Expression of Stat3\|GFP and Dstat3\|NLS\|GFP without IL\|6 stimulation resu lted in predominantly cytoplasm localization in 293T cell. The results suggest that latent Stat3 is not anchored in the cytoplasm, and that nuclear localizatio n in response to IL\|6 is facilitated by gain of an NLS function.