%0 Journal Article
%T The Mechanism of Stat3 Nuclear Import<br>STAT3 入核分子机制的初步研究
%A YE Zhong
%A |De
%A SHEN Bei-Fen SONG Lun
%A <br>叶中德
%A 沈倍奋
%A 宋伦
%J 生物工程学报
%D 2004
%I 
%X In order to investigate the mechanism of stat3 nuclear import. positio ned a characterized NLS of the SV40 large T antigen into Stat3\|GFP ,Dstat3\|GF P respectively between the C\|terminus of Stat3 and the N\|terminus of GFP to cr eate Stat3\|NLS\|GFP and Dstat3\|NLS\|GFP. With NLS\|GFP as the positive contro l, Expression of the Stat3\|NLS\|GFP without IL\|6 stimulation and Stat3\|GFP wi th IL\|6 stimulation resulted in a predominantly nuclear localization in 293T ce ll. Expression of Stat3\|GFP and Dstat3\|NLS\|GFP without IL\|6 stimulation resu lted in predominantly cytoplasm localization in 293T cell. The results suggest that latent Stat3 is not anchored in the cytoplasm, and that nuclear localizatio n in response to IL\|6 is facilitated by gain of an NLS function.
%K Stat3
%K nuclear import
%K NLS<br>Stat3
%K 细胞核
%K 入核分子机制
%K 核定位序列
%K NLS
%K 白介素-6
%K Stat3-NLS-GFP
%K Dstat3-NLS-GFP
%K 融合分子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=6EE186319B849198&yid=D0E58B75BFD8E51C&vid=A04140E723CB732E&iid=0B39A22176CE99FB&sid=BF112261B65CB9C9&eid=DFEE4E8C33C95CEF&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=20