|
|
生物物理学报 1992
EFFECT OF PHENYLHYDRAZINE ON STRUCTURE AND FUNCTION OF ERYTHROCYTE MEMBRANES
|
Abstract:
The membrane-Hb interaction was modified by treating intact erythrocytes with phenylhydrazine. The effect of the modification on structure and function of erythrocyte membrane was studied by measuring osmotic hemolytic process, fluorescent quenching, and anion transport. The fast process (K1) of osmotic hemolysis was affected at phenylhydrazine concentration >0.01 mM. This means that the membrane fluidity is decreased. The binding of Hb to the membrane was increased rapidly at phenylhydnazine concentration > 0.1 mM. Furthermore, we observed that the anion transport across erythrocyte membrane was increased due to change of membrane structure.
