Optimized Pyruvate Production Through Enhancing Torulopsis glabrata Process Performance
丙酮酸发酵过程中光滑球拟酵母过程功能的强化

Based on kinetic parameters analysis, a 4-stage glucose-control strategy was used to optimize pyruvate production by Torulopsis glabrata. At 0~18 h, 40 g/L glucose was fed to get a higher specific growth rate, and then glucose was quickly fed to the fermentation broth at 19~25 h to achieve the maximum dry cell concentration and to redirect the carbon flux from cell growth to pyruvate formation. At 26~63 h, a highest pyruvate yield (0.71 g/g) was achieved through maintaining glucose at 33.4 g/L by a singular control strategy. After 64 h, pyruvate fermentation was continued as batch process. A high concentration (83.1 g/L), yield (0.621 g/g) and productivity (1.00 g/(L·h)) of pyruvate were achieved by applying this strategy, which were 21.3%, 21.6% and 29.9% higher than that of the batch process.