THE EFFECT OF SOLUBLE AND FIBRILAR Aβ1-40 ON MEMBRANE PERMEABILITY
可溶的和纤维化的Aβ1-40对膜脂的通透性的影响

The time course of Aa 1-40 fibril formation was characterized using a variety of assays. The effect of soluble and fibrillar Aa 1-40 on the membrane permeability was assessed by measuring the re-l ease of vesicle-entrapped with three different molecular weight fluoresc ence probes (ANTS/DPX, Calcein, FD-4). The results showed that fibrils had formed after 4 days. Aggregated Aa 1-40 may induce the leakage of ANTS and Calcein in a dose-dependent manner, but soluble Aa 1-40 had no such effect. It suggests that the change of physico-chemi cal properties of membrane constituents may have some relation to Aa neuro-toxicity.