Calcium-regulated Growth and Differentiation of the Mouse Epidermal Keratinocytes
钙离子对鼠角质细胞生长和分化的影响

The effect of the calcium ion (Ca 2+) on the growth and differentiation of the mouse epidermal keratinocytes cultured in serum-free medium was investigated. It was found that the optimal level of calcium ion in the medium was about 0.2mmol/L. Under such a culture condition the colony forming efficiency, attachment percentage, percentage of the cells with cornified envelops, and percentage of the senesced cells were measured to be about 10.8 %, 30.8 %, 5.1 %, and 26.8 %, respectively. However, the Ca 2+ concentrations in the medium above 0.6mmol/L resulted in significant differentiation and senescence of the keratinocytes, which was found to be harmful for keratinocyte growth and expansion in vitro.