%0 Journal Article
%T Calcium-regulated Growth and Differentiation of the Mouse Epidermal Keratinocytes<br>钙离子对鼠角质细胞生长和分化的影响
%A YU Hai-Qiang
%A ZHOU Yan
%A HUA Ping
%A TAN Wen-Song
%A <br>于海强
%A 周燕
%A 华平
%A 谭文松
%J 生物工程学报
%D 2002
%I 
%X The effect of the calcium ion (Ca 2+) on the growth and differentiation of the mouse epidermal keratinocytes cultured in serum-free medium was investigated. It was found that the optimal level of calcium ion in the medium was about 0.2mmol/L. Under such a culture condition the colony forming efficiency, attachment percentage, percentage of the cells with cornified envelops, and percentage of the senesced cells were measured to be about 10.8 %, 30.8 %, 5.1 %, and 26.8 %, respectively. However, the Ca 2+ concentrations in the medium above 0.6mmol/L resulted in significant differentiation and senescence of the keratinocytes, which was found to be harmful for keratinocyte growth and expansion in vitro.
%K keratinocytes
%K cell culture
%K cell differentiation
%K cell senescence<br>角质细胞，
%K 细胞培养，
%K 细胞分化，
%K 细胞老化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=4FBC4BB9A4EF13EA&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=94C357A881DFC066&sid=C824C8F9F54AE9B9&eid=E4EC39E73004B593&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=10