Cloning and Expression of the Cecropin B-thanatin Hybrid Antimicrobial Peptide in Escherichia coli
家蚕抗菌肽-死亡素杂合肽基因在大肠杆菌中的克隆与表达

A 44-residue hybrid peptide (CB (1-24)-Arg-Ser-Tyr-Tan (4-21)) incorporating 1-24 residues of cecropin B (CB) and 4-21 residues of thanatin (Tan) was designed and constructed. The CB-Tan gene was cloned into expression plasmid pGEX-3X and expressed in E.coli BL21. The fusion protein was purified by affinity chromatography. After digested with enterokinase the gene product released with antibacterial activity and gave one band in Tricine-SDS-PAGE.