%0 Journal Article
%T Cloning and Expression of the Cecropin B-thanatin Hybrid Antimicrobial Peptide in Escherichia coli<br>家蚕抗菌肽-死亡素杂合肽基因在大肠杆菌中的克隆与表达
%A WENG Hong-Biao
%A NIU Bao-Long MENG Zhi-Qi XU Meng-Kui
%A <br>翁宏飚
%A 牛宝龙
%A 孟智启
%A 徐孟奎
%J 生物工程学报
%D 2002
%I 
%X A 44-residue hybrid peptide (CB (1-24)-Arg-Ser-Tyr-Tan (4-21)) incorporating 1-24 residues of cecropin B (CB) and 4-21 residues of thanatin (Tan) was designed and constructed. The CB-Tan gene was cloned into expression plasmid pGEX-3X and expressed in E.coli BL21. The fusion protein was purified by affinity chromatography. After digested with enterokinase the gene product released with antibacterial activity and gave one band in Tricine-SDS-PAGE.
%K cecropin B
%K thanatin
%K hybrid peptide
%K fusion expression<br>家蚕
%K 抗菌肽－死亡素杂合肽基因
%K 大肠杆菌
%K 克隆
%K 表达
%K Cec
%K B－Tan基因
%K 融合表达
%K 抗菌活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=E8130D8DFB447F9C&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=38B194292C032A66&sid=EA64BD0B2FF6E786&eid=26AEEED215BE97D0&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=19