THE MOLECULAR ANALYSIS OF CABBAGE TOTAL DNA TRANSFORMATION INTO A.thaliana MEDIATED BY keV ION BEAM
离子束介导甘蓝全DNA转化拟南芥菜的分子分析

The total DNA of cabbage was transferred into A. thaliana mediated by Ar+ ion beam with the energy of 30 keV and influence of 1.5×1017 ions/cm2. Among the 94 transferred plants, there were 6 phenotypic variation plants. One of them, marked as T-5, was studied. The genomes of T-5 and its offspring were analyzed by RAPD-PCR with 80 10-base random primers. The result showed that by contrast to the control, a new band, T-5S176-620, was amplified from T-5 and its offspring T-5-2 with random primer S176. Its sequence was aligned with genome of A. thaliana by means of program of homologous alignment. A Southern blot to cabbage genome with T-5S176-620 as probe was carried out. Both of their results indicated that T-5S176-620 was not in A. thaliana but from cabbage. However, the desired length DNA segments could not be amplified from cabbage genome with primers designed with reference to T-5S176-620. In terms of the specialties of total DNA transformation mediated by keV ion beam, the possible reason was analyzed.