%0 Journal Article
%T THE MOLECULAR ANALYSIS OF CABBAGE TOTAL DNA TRANSFORMATION INTO A.thaliana MEDIATED BY keV ION BEAM<br>离子束介导甘蓝全DNA转化拟南芥菜的分子分析
%A BIAN Po
%A SU Ming-jie
%A QIN Guang-yong
%A YU Zeng-liang
%A HUO Yu-ping
%A <br>卞坡
%A 苏明杰
%A 秦广雍
%A 余增亮
%A 霍裕平
%J 生物物理学报
%D 2003
%I 
%X The total DNA of cabbage was transferred into A. thaliana mediated by Ar+ ion beam with the energy of 30 keV and influence of 1.5×1017 ions/cm2. Among the 94 transferred plants, there were 6 phenotypic variation plants. One of them, marked as T-5, was studied. The genomes of T-5 and its offspring were analyzed by RAPD-PCR with 80 10-base random primers. The result showed that by contrast to the control, a new band, T-5S176-620, was amplified from T-5 and its offspring T-5-2 with random primer S176. Its sequence was aligned with genome of A. thaliana by means of program of homologous alignment. A Southern blot to cabbage genome with T-5S176-620 as probe was carried out. Both of their results indicated that T-5S176-620 was not in A. thaliana but from cabbage. However, the desired length DNA segments could not be amplified from cabbage genome with primers designed with reference to T-5S176-620. In terms of the specialties of total DNA transformation mediated by keV ion beam, the possible reason was analyzed.
%K Total
%K DNA
%K transformation
%K mediated
%K by
%K keV
%K Ar~+
%K ion
%K beam
%K Arabidopsis thaliana
%K Randomly
%K amplified
%K polymorphic
%K DNA
%K Homologous
%K alignment<br>离子束
%K 介导
%K 甘蓝
%K DNA
%K 转化
%K 拟南芥菜
%K 分子分析
%K 同源比对
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=395060DA58A7FDDA&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=38B194292C032A66&sid=170CE8B011EA4FD9&eid=2E01F39B6CBD53DE&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=8&reference_num=15