The Cloning, Sequence Analysis and Expression in Escherichia coli of Coat Protein Gene of Grapevine Fanleaf Virus
葡萄扇叶病毒外壳蛋白基因的克隆,序列分析及其在大肠杆菌中的表达

Fragments of 5' end and 3' end of GFLV's coat protein gene (CP gene) were amplified separately through RT-PCR. The two products were cloned into vector pGEM-5Zf ( ) and ligated into a whole CP gene via Bgl I restriction site. The DNA sequence of this gene has been determined, which contains 1512bp and encodes 504 amino acid residues. Comparison with strain GFLV-F13 shows 88. 4% homology in nu-cleotides, and 95. 8% similarity in amino acid residues. This CP gene has got expression in E. coli.