%0 Journal Article
%T The Cloning, Sequence Analysis and Expression in Escherichia coli of Coat Protein Gene of Grapevine Fanleaf Virus<br>葡萄扇叶病毒外壳蛋白基因的克隆,序列分析及其在大肠杆菌中的表达
%A Guan Hancheng Cai Wenqi Mang Keqiang
%A <br>管汉成
%A 蔡文启
%A 莽克强
%J 生物工程学报
%D 1996
%I 
%X Fragments of 5' end and 3' end of GFLV's coat protein gene (CP gene) were amplified separately through RT-PCR. The two products were cloned into vector pGEM-5Zf ( ) and ligated into a whole CP gene via Bgl I restriction site. The DNA sequence of this gene has been determined, which contains 1512bp and encodes 504 amino acid residues. Comparison with strain GFLV-F13 shows 88. 4% homology in nu-cleotides, and 95. 8% similarity in amino acid residues. This CP gene has got expression in E. coli.
%K Grapevine fanleaf virus (GFLV)
%K coat protein gene
%K RT-PCR<br>葡萄扇叶病毒
%K 外壳蛋白基因
%K RT-PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C37A6DD7168AC1CA7DEC8942F0813871&yid=8A15F8B0AA0E5323&vid=59906B3B2830C2C5&iid=0B39A22176CE99FB&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=0