The Effect of Glucose Addition on the Suspension Growth of Insect Cell Sf21
葡萄糖的添加对昆虫细胞Sf21悬浮生长的影响

It was studied the effect of glucose addition on the suspension growth of insect cell Sf21. Compared with no glucose addition, it was found that at the addition of Ig/L, the growth rate of Sf21 was enhanced, the maximal density was increased from 2. 5 x 106/ml to 4- 9x106/ml; at the addition of 2g/L, the inhibition effect appeared, even the inoculum density was increased, the maximal density of Sf21 cell was only 2.1 x 106/ml. When the glucose-fed batch culture was used, the maximal density of Sf21 culture could be more increased to 5. 2x106/ml. In the compare of glucose and lactate metabolism under these conditions, it was found that at the addition of 2g/L, the metabolism of glucose and lactate was different from other conditions. However, it was difficult to explain the inhibition effect of the glucose at high concentration.