%0 Journal Article
%T The Effect of Glucose Addition on the Suspension Growth of Insect Cell Sf21<br>葡萄糖的添加对昆虫细胞Sf21悬浮生长的影响
%A Li Wenqing Xiao Chengzu Huang Zicai Chen Zhaolie
%A <br>李文青
%A 肖成祖
%A 黄子才
%A 陈昭烈
%J 生物工程学报
%D 1996
%I 
%X It was studied the effect of glucose addition on the suspension growth of insect cell Sf21. Compared with no glucose addition, it was found that at the addition of Ig/L, the growth rate of Sf21 was enhanced, the maximal density was increased from 2. 5 x 106/ml to 4- 9x106/ml; at the addition of 2g/L, the inhibition effect appeared, even the inoculum density was increased, the maximal density of Sf21 cell was only 2.1 x 106/ml. When the glucose-fed batch culture was used, the maximal density of Sf21 culture could be more increased to 5. 2x106/ml. In the compare of glucose and lactate metabolism under these conditions, it was found that at the addition of 2g/L, the metabolism of glucose and lactate was different from other conditions. However, it was difficult to explain the inhibition effect of the glucose at high concentration.
%K Insect cell culture
%K glucose
%K Sf21<br>昆虫细胞培养，葡萄糖，Sf21
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EF90B7E4FF70CC9780A41473A269DD2C&yid=8A15F8B0AA0E5323&vid=59906B3B2830C2C5&iid=CA4FD0336C81A37A&sid=771152D1ADC1C0EB&eid=8E6AB9C3EBAAE921&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=1