GTPγs MODULATION OF SAIKOSAPONIN(I) STIMULATED AMYLASE SECRETION IN ISOLATED PANCREATIC ACINI
GTPγs对柴胡皂甙(I)刺激胰腺腺泡酶分泌的影响

To elucidate the site of action of Saikosaponin (I) in pancreatic acinar cells, the modulatory effects of guanosine 5'-triphosphate(GTPγs) were investigated in SLO permeabilized pancreatic acinar cells. In a permeabilizing medium, GTPγs addition induced 15min amylase secretion, the effects being maximal at 10-7mol·L-1. GTPγs effects were concentration dependent. GTPγs 10-7mol·L-1 increased amylase secretion stimulated by 10-5mol·L-1 SA(I) 1.6 fold. After acinar cells were pre-permeabilized with SLO for 10min, GTPγs shifted the dose-response curve of SA(I) stimulated amylase secretion to the left, decreasing EC50 from 2.0×10-5 to 1.0×10-5mol·L-1. These results suggest receptor-coupled G protein activation is involved during SA(I) stimulation of amylase secretion.