%0 Journal Article
%T GTPγs MODULATION OF SAIKOSAPONIN(I) STIMULATED AMYLASE SECRETION IN ISOLATED PANCREATIC ACINI<br>GTPγs对柴胡皂甙(I)刺激胰腺腺泡酶分泌的影响
%A WANG Hui
%A YU Yi
%A ZHANG Wen-zheng
%A DONG Zhi-yong
%A LIU Bao-hua
%A YANG Wen-xiu
%A <br>王辉
%A 余奕
%A 张文正
%A 董志勇
%A 刘保华
%A 杨文修
%J 生物物理学报
%D 2001
%I 
%X To elucidate the site of action of Saikosaponin (I) in pancreatic acinar cells, the modulatory effects of guanosine 5'-triphosphate(GTPγs) were investigated in SLO permeabilized pancreatic acinar cells. In a permeabilizing medium, GTPγs addition induced 15min amylase secretion, the effects being maximal at 10-7mol·L-1. GTPγs effects were concentration dependent. GTPγs 10-7mol·L-1 increased amylase secretion stimulated by 10-5mol·L-1 SA(I) 1.6 fold. After acinar cells were pre-permeabilized with SLO for 10min, GTPγs shifted the dose-response curve of SA(I) stimulated amylase secretion to the left, decreasing EC50 from 2.0×10-5 to 1.0×10-5mol·L-1. These results suggest receptor-coupled G protein activation is involved during SA(I) stimulation of amylase secretion.
%K Saikosaponin(I)
%K GTP&gamma
%K s
%K Pancreatic acini
%K Amylase secretion
%K G protein<br>柴胡皂甙
%K GTPγs
%K 胰腺腺泡细胞
%K 酶分泌
%K G蛋白
%K 信号传导
%K 膜受体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=4FB7327D160006B0&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=E158A972A605785F&sid=821800203AD09E7B&eid=250DF325A002B9CC&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=1&reference_num=10