Isolation and Identification of Reprogramming Genes Related to the Development of the Rabbit Reconstructed Embryos
兔移核重构胚再程序化相关基因片段的分离与鉴定

By the method of single preimplantation embryos differential display polymerase chain reaction (SPEDDRT PCR),25 reprogramming cDNA fragments were obtained from single 2 cell,8 cell embryos and blastula. After cloning and sequencing, five of them were identified by reverse Northern and characterized with stage specific expression during reconstructed embryo development. This results will help to isolate full length reprogramming genes and study their function during embryonic development.