%0 Journal Article
%T Isolation and Identification of Reprogramming Genes Related to the Development of the Rabbit Reconstructed Embryos<br>兔移核重构胚再程序化相关基因片段的分离与鉴定
%A YU Wei
%A Dong
%A LI Wen
%A Yong
%A WANG Yu
%A Ge
%A YANG Li
%A Xin
%A LIU Gui
%A Sheng
%A DU Miao
%A CHEN Qing
%A Xuan
%A <br>郁卫东
%A 李文雍
%A 王玉阁
%A 杨立新
%A 刘桂生
%A 杜淼
%A 陈清轩
%J 生物工程学报
%D 2003
%I 
%X By the method of single preimplantation embryos differential display polymerase chain reaction (SPEDDRT PCR),25 reprogramming cDNA fragments were obtained from single 2 cell,8 cell embryos and blastula. After cloning and sequencing, five of them were identified by reverse Northern and characterized with stage specific expression during reconstructed embryo development. This results will help to isolate full length reprogramming genes and study their function during embryonic development.
%K cloning
%K preimplantation embryos
%K single embryos mRNA differential display
%K reprogramming<br>克隆，
%K 植入前胚胎，
%K 单个植入前胚胎mRNA差别显示，
%K 再程序化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A340800D6944F1B5&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=CA4FD0336C81A37A&sid=340AC2BF8E7AB4FD&eid=339D79302DF62549&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=22