|
|
生物工程学报 1999
Chimeric Antibody Production in Silkworm Cells and Silkworm Larvae
|
Abstract:
By using recombinant Baculovirus expression system,the anti human small cell lung cancer chimeric antibody had been expressed in Bombyx mori N (BmN) cellsand silkworm larva. The chimeric antibodies which were produced in BmN cells, in larva infected with recombinant viruses rNPVL2,rNPVH17,and dualrecombinant virus rNPVLH19 could be detected by Western blot analysis. The coexpression products of heavy and light chain genes in silkworm larva revealed more strong immune binding activity than single heavy or light chain gene expression.
