%0 Journal Article
%T Chimeric Antibody Production in Silkworm Cells and Silkworm Larvae<br>家蚕细胞和虫体产生抗人小细胞肺癌抗体
%A Yang Guanzhen
%A Wu Xiangfu
%A <br>杨冠珍
%A 吴祥甫
%A 刘健
%A 张中华
%J 生物工程学报
%D 1999
%I 
%X By using recombinant Baculovirus expression system,the anti human small cell lung cancer chimeric antibody had been expressed in Bombyx mori N (BmN) cellsand silkworm larva. The chimeric antibodies which were produced in BmN cells, in larva infected with recombinant viruses rNPVL2,rNPVH17,and dualrecombinant virus rNPVLH19 could be detected by Western blot analysis. The coexpression products of heavy and light chain genes in silkworm larva revealed more strong immune binding activity than single heavy or light chain gene expression.
%K Small cell lung cancer
%K chimeric antibody
%K dual
%K recombinant virus
%K gene expression<br>小细胞肺癌嵌合抗体，双重组家蚕病毒，基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=15C48A8162FEBD2A2FB437265B8BC0F4&yid=B914830F5B1D1078&vid=23CCDDCD68FFCC2F&iid=CA4FD0336C81A37A&sid=2001E0D53B7B80EC&eid=94E7F66E6C42FA23&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=6