Direct Embryogenesis from Protoplast of Winter Wheat
冬小麦原生质体培养的胚状体直接发生

Friable embryogenic calli were obtained on a modified N medium (NBD medium) from a winter wheat cultivar "Jinghua No.1"(Triticum aestivurn L. cu. Jinghua No.1) and were transferred to a modified MS liquid medium (MSDL medium) to initiate embryogenic suspension cultures. Protoplasts were isolated from the suspensions and cultured on a modified MS medium (MSDP medium). The somatic embryoids were formed directly from the protoplasts and germinated into entire plants. The development of the somatic embryoids was very similar to that of zygotic embryos of wheat.