%0 Journal Article
%T Direct Embryogenesis from Protoplast of Winter Wheat<br>冬小麦原生质体培养的胚状体直接发生
%A GE Tai-Ming
%A ZHANG Rong-De
%A QIN Fa-Lan
%A YU Yu-Jun
%A XIE Yue-Feng
%A <br>葛台明
%J 生物工程学报
%D 2000
%I 
%X Friable embryogenic calli were obtained on a modified N medium (NBD medium) from a winter wheat cultivar "Jinghua No.1"(Triticum aestivurn L. cu. Jinghua No.1) and were transferred to a modified MS liquid medium (MSDL medium) to initiate embryogenic suspension cultures. Protoplasts were isolated from the suspensions and cultured on a modified MS medium (MSDP medium). The somatic embryoids were formed directly from the protoplasts and germinated into entire plants. The development of the somatic embryoids was very similar to that of zygotic embryos of wheat.
%K Winter wheat (Triticum aestivum L
%K )
%K Jinghua No
%K 1
%K protoplast culture
%K direct somatic embryogenesis
%K plant regeneration<br>冬小麦，京花一号，原生质体培养，体细胞胚胎直接发生，植株再生
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=2F365BCE5E49E9D2&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=94C357A881DFC066&sid=5568599C60D4BE87&eid=9E7C0CB25117E09B&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=10