The Effects of Different Glucose Feeding Modes on hEGF Production in an Excretory Recombinant Escherichia coli K12 System
不同流加发酵方式对重组大肠杆菌细胞外Hegf表达水平的影响

The effects of different glucose feeding modes on hEGF expression were evaluated in an excretory recombinant \%E.coli\% K12 system.The results showed that,compared with batch cultivation,the plasmid stability and density of plasmid\|retaining cells were improved by all three glucose feeding modes (intermittent,pH\|stat and constant\|rate).It was shown that hEGF yields were improved up to 25.5% and 28.1% by intermittent or pH\|stat glucose feeding respectively.Especially,up to 150% improvement of hEGF production was achieved by constant feeding of 200g/L glucose solution at a rate of 0.11 mL/min.The effects of further combined feeding with other medium components (ampicillin,nitrogen sources,and inorganic salts) and inducer on hEGF yield were also examined in the bench\|top fermentor.