%0 Journal Article
%T The Effects of Different Glucose Feeding Modes on hEGF Production in an Excretory Recombinant Escherichia coli K12 System<br>不同流加发酵方式对重组大肠杆菌细胞外Hegf表达水平的影响
%A XU Zhi
%A |Nan
%A CEN Pei
%A |Lin
%A W K R Wong
%A <br>徐志南
%A 岑沛霖
%A W K R Wong
%J 生物工程学报
%D 2001
%I 
%X The effects of different glucose feeding modes on hEGF expression were evaluated in an excretory recombinant \%E.coli\% K12 system.The results showed that,compared with batch cultivation,the plasmid stability and density of plasmid\|retaining cells were improved by all three glucose feeding modes (intermittent,pH\|stat and constant\|rate).It was shown that hEGF yields were improved up to 25.5% and 28.1% by intermittent or pH\|stat glucose feeding respectively.Especially,up to 150% improvement of hEGF production was achieved by constant feeding of 200g/L glucose solution at a rate of 0.11 mL/min.The effects of further combined feeding with other medium components (ampicillin,nitrogen sources,and inorganic salts) and inducer on hEGF yield were also examined in the bench\|top fermentor.
%K human epidermal growth factor
%K recombinant E
%K coli
%K fed\|batch cultivation
%K glucose feeding<br>人表皮生长因子
%K 重组大肠杆菌
%K 流加发酵
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=DA22707EA50DE235&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=94C357A881DFC066&sid=65FC738C50B41E43&eid=0A8675156EB60B87&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=13