Study on Hypocotyl Protoplast Culture of Rapeseed
甘蓝型油菜下胚轴原生质体培养的研究

Protoplasts were isolated from hypocotyl of two rapeseed (Brassica napus L. )cultivars "Yunbei 2" and "Ningyou 7". Light treatment during seed germination could apparently increase the division frequency. The purified protoplasts were cultured with three different methods. The method called "agarose island" was found superior to the two others ("thin liquid layer" and "agarose solid layer"). Protoplasts cultured in the "agarose islane" divided more rapidly, and developed into calli more frequently. Shoot regeneration occurred when the protoplasts-derived calli were transferred onto differentiation medium. Individual shoot was rooted on the rooting medium. Whole plants have been transplanted into pots,and grow well in the phytotron. The "agarose island" method might be suited to the genetic transformation of protoplasts because the liquid medium surrlunding the "island" could be conveniently replaced without disturbing the microenvironment of protoplasts plated.