%0 Journal Article
%T Study on Hypocotyl Protoplast Culture of Rapeseed<br>甘蓝型油菜下胚轴原生质体培养的研究
%A Cheng Zhendong Wei Zhiming Xu Zhihong
%A <br>程振东
%A 卫志明
%A 许智宏
%J 生物工程学报
%D 1994
%I 
%X Protoplasts were isolated from hypocotyl of two rapeseed (Brassica napus L. )cultivars "Yunbei 2" and "Ningyou 7". Light treatment during seed germination could apparently increase the division frequency. The purified protoplasts were cultured with three different methods. The method called "agarose island" was found superior to the two others ("thin liquid layer" and "agarose solid layer"). Protoplasts cultured in the "agarose islane" divided more rapidly, and developed into calli more frequently. Shoot regeneration occurred when the protoplasts-derived calli were transferred onto differentiation medium. Individual shoot was rooted on the rooting medium. Whole plants have been transplanted into pots,and grow well in the phytotron. The "agarose island" method might be suited to the genetic transformation of protoplasts because the liquid medium surrlunding the "island" could be conveniently replaced without disturbing the microenvironment of protoplasts plated.
%K Brassica napus L
%K protoplasts culture
%K plant regeneration<br>甘蓝型油菜，原生质体培养，檀株再生
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0795B99E992E80EE65FED146BCC07635&yid=3EBE383EEA0A6494&vid=F3090AE9B60B7ED1&iid=CA4FD0336C81A37A&sid=340AC2BF8E7AB4FD&eid=27746BCEEE58E9DC&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=13&reference_num=7