Construction and Expression of Coexpression Plasmid of Carcinoembryonic Antigen and Newcastle Disease virus HN Gene
人癌胚抗原与新城疫病毒HN基因共表达载体的构建及表达

The eukaryotic expression plasmid (pcDNA3-CEA)containing with the gene coding for the Carcinoembryonic antigen(CEA) had been gotten with RT-PCR and gene recombination techniques. Using enzymolysis, ligation and other techniques, an eukaryotic coexpression plasmid(pcDNA3-CEA/HN)containing two expression unites of CEA and Newcastle disease virus HN gene that may have the function of immunoenhancement had been constructed. The plasmid will lay a foundation for further researching CEA nucleiotide vaccine, adjuvant and their effect of special antitumor immune.