Studies on Expression and Function of Human Membrane Cofactor Protein in NIH3T3 Cells
人MCP cDNA在NIH3T3细胞中表达及功能研究

One recombinant human membrane cofactor protein (MCP) expression plasmid pcDNA3 MCP was constructed.After transfected into NIH3T3 cells with calcium phosphate DNA precipitate method,the expression clones were obtained by G418 selection.To determine whether MCP generated on heterologous cells by cDNA transfection retains its capacity to inhibit human complement,NIH3T3 cells transfected with MCP cDNA were incubated with human serum as well as guinea pig,rabbit serum.Under identical conditions,the control cells transfected with pcDNA3 vector were completely lysed by human serum but the cells transfected with MCP were barely lysed.However,the cells transfected with MCP were lysed by guinea pig and rabbit serum complement as the same extent as control cells.