%0 Journal Article
%T Studies on Expression and Function of Human Membrane Cofactor Protein in NIH3T3 Cells<br>人MCP cDNA在NIH3T3细胞中表达及功能研究
%A HUANG Jian
%A LI Wen
%A Xin
%A ZHEN Cong
%A Yi
%A <br>黄健
%A 李文鑫
%A 郑从义
%J 生物工程学报
%D 2000
%I 
%X One recombinant human membrane cofactor protein (MCP) expression plasmid pcDNA3 MCP was constructed.After transfected into NIH3T3 cells with calcium phosphate DNA precipitate method,the expression clones were obtained by G418 selection.To determine whether MCP generated on heterologous cells by cDNA transfection retains its capacity to inhibit human complement,NIH3T3 cells transfected with MCP cDNA were incubated with human serum as well as guinea pig,rabbit serum.Under identical conditions,the control cells transfected with pcDNA3 vector were completely lysed by human serum but the cells transfected with MCP were barely lysed.However,the cells transfected with MCP were lysed by guinea pig and rabbit serum complement as the same extent as control cells.
%K Expression of MCP cDNA
%K NIH3T3 cell
%K complement lysis<br>同源限制因子
%K MCP
%K NIG3T3细胞
%K 补体溶破
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=FDDE5F9CDF9A40A5&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=0B39A22176CE99FB&sid=7CE3F1F20DE6B307&eid=0B4F496D54044D86&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=10