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生物工程学报 1998
Construction of Engineering Host by Non random Method in Sacchromyces cerevisiae
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Abstract:
A yeast engineering host,GJ30,was construction by nonrandom method.GJ30 derivated from wild type yeast have leu 2 - and ura 3 - deficients which can be used as selecting markers.The PCR analysis showed that the homologous recombinations were happened in the leu2 and ura3 locus of GJ30 respectively.The biological properties (cell growth density,stability and efficiency for gene expression) of GJ30 were compared with other several yeast tains.The results showed that GJ30 is a suitable host strain for expression of foreign genes.
