%0 Journal Article
%T Construction of Engineering Host by Non random Method in Sacchromyces cerevisiae<br>非随机方法构建酿酒酵母基因工程菌
%A Gong Yi
%A Chen Yan
%A Yang Shengli
%A <br>龚毅
%A 陈燕
%A 杨胜利
%J 生物工程学报
%D 1998
%I 
%X A yeast engineering host,GJ30,was construction by nonrandom method.GJ30 derivated from wild type yeast have leu 2 - and ura 3 - deficients which can be used as selecting markers.The PCR analysis showed that the homologous recombinations were happened in the leu2 and ura3 locus of GJ30 respectively.The biological properties (cell growth density,stability and efficiency for gene expression) of GJ30 were compared with other several yeast tains.The results showed that GJ30 is a suitable host strain for expression of foreign genes.
%K Yeast
%K host strain
%K recombination<br>酿酒酵母
%K 基因工程菌
%K 构建
%K 非随机法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=362D5F09D03AD55CC54C2987B307C28D&yid=8CAA3A429E3EA654&vid=F3583C8E78166B9E&iid=38B194292C032A66&sid=2E01F39B6CBD53DE&eid=B66C5792F4740920&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=1