3-D RECONSTRUCTION OF VISUAL MICROSTRUCTURES BY MEANS OF A CONFOCAL MICROSCOPE SYSTEM
利用共聚焦显微镜系统进行视觉显微结构的三维重建

In this paper 3-D reconstruction of three kinds of visual microstructures by means of a confocal laser scan microscope system is introduced. The specimens for reconstruction are: neurons in optic tectum of pigeon, intertectal commissures of toad and the crystaiine rones of ommalidia in dragonfly. The serial section images for computer 3-D reconstruction are from confocal optical section at 1 or 3 ?m intervals. The results of 3-D reconstruction are satisfactory for their true object and stereoscopic sensation, especially that of the specimen stained by a fluorescent dye. This is the first time that the 3-D morphology of the visual microstructures is exhibited. The principle of the instrument and its advantages compared with conventional microscope are also introduced.