Decolorization and Isolation of Recombinant Hirudin Expressed in the Methylotrophic Yeast Pichia pastoris
毕赤酵母发酵液的脱色和重组水蛭素的分离

Schemes for decolorization of broth and isolation of recombinant hirudin from the fermentation supernatants of recombinant yeast %Pichia pastoris% were developed to remove a great deal of non-desired proteins and coloring matters.Based on the stability of hirudin,heating is desirable to pre-treat the fermentation broth.Several different column chromatographies,including anion-exchange,cation-exchange,hydrokyapatite adsorption,hydrophobic interaction,gel filtration and their combination were investigated on the efficiencies of decolorization and isolation.Among these methods,the combination of cation-exchange and anion-exchange was found to be a success in both decolorization and isolation of hirudin.