%0 Journal Article
%T Decolorization and Isolation of Recombinant Hirudin Expressed in the Methylotrophic Yeast Pichia pastoris<br>毕赤酵母发酵液的脱色和重组水蛭素的分离
%A ZHOU Wei-Bin ZHOU Xiang-Shan ZHANG Yuan-Xing
%A <br>周卫斌
%A 周祥山
%A 张元兴
%J 生物工程学报
%D 2001
%I 
%X Schemes for decolorization of broth and isolation of recombinant hirudin from the fermentation supernatants of recombinant yeast %Pichia pastoris% were developed to remove a great deal of non-desired proteins and coloring matters.Based on the stability of hirudin,heating is desirable to pre-treat the fermentation broth.Several different column chromatographies,including anion-exchange,cation-exchange,hydrokyapatite adsorption,hydrophobic interaction,gel filtration and their combination were investigated on the efficiencies of decolorization and isolation.Among these methods,the combination of cation-exchange and anion-exchange was found to be a success in both decolorization and isolation of hirudin.
%K recombinant hirudin
%K decolorization
%K isolation<br>重组水蛭素
%K 脱色
%K 分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C85F4EF1A583ADD2&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=B31275AF3241DB2D&sid=21A4BC96BDC43D33&eid=F4C2D192FB73A21F&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=9&reference_num=15