全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

Studies on the Cell Suspension Culture of Saussarea medusa in a Stirred Tank Bioreactor
搅拌式生物反应器悬浮培养水母雪莲细胞的研究

Keywords: Saussarea medusa,bioreactor,suspension culture,flavonoids
水母雪莲
,生物反应器,植物细胞,悬浮培养,黄酮

Full-Text   Cite this paper   Add to My Lib

Abstract:

The cell suspension culture of Saussarea medusa in a 2L aerated and agitated bioreactor with a four-pitch-blade impeller was investigated. The effects of agitation speed, aeration and inoculum size on cell growth and flavonoids production were studied and it was found that cells had optimum growth and flavonoids production when cultivated at 75 r/min, 700-1000 L/min and an inoculum of 4.0-5.0 g/L. A high cell biomass of 13.8 g/L and flavonoids production of 416 mg/L were achieved after 12 days of cultivation. Time course study revealed that flavonoids biosynthesis was growth-associated. The studies on aggregates size distribution in the bioreactor showed that the aggregates break-up caused by hydrodynamic stress might adversely affect cell growth and lead to significant reduction of cell biomass and flavonoids production.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133