%0 Journal Article
%T Studies on the Cell Suspension Culture of Saussarea medusa in a Stirred Tank Bioreactor<br>搅拌式生物反应器悬浮培养水母雪莲细胞的研究
%A Y Huang
%A D X Zhao
%A D P Lu
%A F Yan
%A Z H Li
%A H Z Chen
%A Q Zhao
%A <br>黄艳
%A 赵德修
%A 吕东平
%A 颜芳
%A 李佐虎
%A 陈洪章
%A 赵乔
%J 生物工程学报
%D 2001
%I 
%X The cell suspension culture of Saussarea medusa in a 2L aerated and agitated bioreactor with a four-pitch-blade impeller was investigated. The effects of agitation speed, aeration and inoculum size on cell growth and flavonoids production were studied and it was found that cells had optimum growth and flavonoids production when cultivated at 75 r/min, 700-1000 L/min and an inoculum of 4.0-5.0 g/L. A high cell biomass of 13.8 g/L and flavonoids production of 416 mg/L were achieved after 12 days of cultivation. Time course study revealed that flavonoids biosynthesis was growth-associated. The studies on aggregates size distribution in the bioreactor showed that the aggregates break-up caused by hydrodynamic stress might adversely affect cell growth and lead to significant reduction of cell biomass and flavonoids production.
%K Saussarea medusa
%K bioreactor
%K suspension culture
%K flavonoids<br>水母雪莲
%K 生物反应器
%K 植物细胞
%K 悬浮培养
%K 黄酮
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=233E33ACB7B4398F&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=94C357A881DFC066&sid=B4F9D541F855CF96&eid=90075EB19043D533&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=7&reference_num=18