Expression in Escherichia coli and Imnunogenicity of Rotavirus VP7
轮状病毒VP7基因在大肠杆菌中的表达及其免疫原性

Rotaviruses are the single most important cause of severe diarrhea in young children all over the world.VP7 is the major outer capsid and is a primary candidate for inclusion in a subunit or recombinant vaccine.Part of the VP7 gene containing all the three antigenic regions was expressed as a chimeric protein with glutathione S\|transferase (GST) in \%E.coli.\%The chimeric protein,representing about 30% of the total protein of the recombinant\|plasmid\|carrying bacteria,reacted with polyclonal antibodies raised against whole virus.Immunization of sero\|negative rabbits and mice with purified fusion\|protein generated both virus\|binding and neutralizing antibodies.