%0 Journal Article
%T Expression in Escherichia coli and Imnunogenicity of Rotavirus VP7<br>轮状病毒VP7基因在大肠杆菌中的表达及其免疫原性
%A YUAN Li
%A |Yong
%A LIU Yong
%A LI Chun
%A |Hong
%A SUN Mao
%A |Sheng
%A DAI Chang
%A |Bai
%A <br>袁力勇
%A 刘勇
%A 李春宏
%A 孙茂盛
%A 戴长柏
%J 生物工程学报
%D 2001
%I 
%X Rotaviruses are the single most important cause of severe diarrhea in young children all over the world.VP7 is the major outer capsid and is a primary candidate for inclusion in a subunit or recombinant vaccine.Part of the VP7 gene containing all the three antigenic regions was expressed as a chimeric protein with glutathione S\|transferase (GST) in \%E.coli.\%The chimeric protein,representing about 30% of the total protein of the recombinant\|plasmid\|carrying bacteria,reacted with polyclonal antibodies raised against whole virus.Immunization of sero\|negative rabbits and mice with purified fusion\|protein generated both virus\|binding and neutralizing antibodies.
%K rotavirus
%K VP7
%K gene expression
%K E
%K coli
%K immunogenicity<br>轮状病毒，
%K VP7，
%K 大肠杆菌，
%K 免疫原性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=520C7147A519247C&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=769BD58726D66E7D&eid=EB552E4CFC85690B&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=9&reference_num=13