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生物工程学报 2000
Expression and Characterization of HIV-1 Gag p~(17-)p~(24) Protein
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Abstract:
The biological and immunological characteristics of HIV 1 core protein p17 24 expressed by recombinant vaccinia viruses were studied.The results of indirect immunofluorescence assay (I IFA),Western blot and dot ELISA showed that the two recombinants could express the p24 gag and p17 24 gag fusion proteins in infected cell lines respectively.The electromicroscope observation revealed that the expressed proteins could also assemble into virus like particles.The recombinant vaccinia viruses can also stimulate mice for the formation of anti HIV 1 Gag p24 antibody.When infected with the recombinant viruses,the chromosome DNA ladder caused by the apoptosis of the BHK cell was observed.
