%0 Journal Article
%T Expression and Characterization of HIV-1 Gag p~(17-)p~(24) Protein<br>HIV-1 Gag p17-p24在痘苗病毒中的表达及性质研究
%A LI Ti yuan
%A JIN Ning yi
%A WANG Hong wei
%A GUO Zhi ru
%A FANG Hou hua
%A AN Ru guo
%A YIN Zhen
%A <br>李体远
%A 金宁一
%A 王宏伟
%A 郭志儒
%A 方厚华
%A 安汝国
%A 殷震
%J 生物工程学报
%D 2000
%I 
%X The biological and immunological characteristics of HIV 1 core protein p17 24 expressed by recombinant vaccinia viruses were studied.The results of indirect immunofluorescence assay (I IFA),Western blot and dot ELISA showed that the two recombinants could express the p24 gag and p17 24 gag fusion proteins in infected cell lines respectively.The electromicroscope observation revealed that the expressed proteins could also assemble into virus like particles.The recombinant vaccinia viruses can also stimulate mice for the formation of anti HIV 1 Gag p24 antibody.When infected with the recombinant viruses,the chromosome DNA ladder caused by the apoptosis of the BHK cell was observed.
%K HIV
%K 1
%K Gag
%K vaccinia virus<br>HIV-1
%K Gag
%K 痘苗病毒
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0599AEE00B40F94C5F30A2CF878D35FE&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=CA4FD0336C81A37A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0