Separation and Purification of Taxol Using Normal-and Reversed-phase Chromatography in Tandem
正相和反相柱层析组合分离纯化紫杉醇

A method is described for separation and purification of taxol from the extract of Tascus cuspidate callus cul-ture with Al2O3 normal-phase chromatography and C18 reversed-phase chromatography, The operating conditions of the two chromatographic steps were investigated and optimized. The content of taxol was greatly increased after Al2O3 chro-matography. The purity of taxol reached 95 % from less than 1.0 % after the two chromatographic steps. The final purity of taxol was more than 98% after the process of crystallization. The 13CNMR spectrum of the product was identical to that of taxol reported in the literature.