%0 Journal Article
%T Separation and Purification of Taxol Using Normal-and Reversed-phase Chromatography in Tandem<br>正相和反相柱层析组合分离纯化紫杉醇
%A ZHANG Zhi-qiang
%A SU Zhi-guo
%A <br>张志强
%A 苏志国
%J 生物工程学报
%D 2000
%I 
%X A method is described for separation and purification of taxol from the extract of Tascus cuspidate callus cul-ture with Al2O3 normal-phase chromatography and C18 reversed-phase chromatography, The operating conditions of the two chromatographic steps were investigated and optimized. The content of taxol was greatly increased after Al2O3 chro-matography. The purity of taxol reached 95 % from less than 1.0 % after the two chromatographic steps. The final purity of taxol was more than 98% after the process of crystallization. The 13CNMR spectrum of the product was identical to that of taxol reported in the literature.
%K Taxol
%K normal-phase column chromatography
%K separation and purification<br>紫杉醇，正相柱层析，分离纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=460D049FF60C7B92&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=CA4FD0336C81A37A&sid=CB423C9A71560A74&eid=B9704B40A4225A24&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=6&reference_num=11