Cloning and Function Determination of Promoter Region of Glucoamylase Gene form Aspergillus niger T21
黑曲霉糖化酶基因启动子区的克隆及其功能测定

A 850bp fragment of the 5' flanking region of the glucoamylase gene was synthesized from A. niger T21 genome using PCR. The function detection vector was constructed by fusing this fragment to E. colt hygromycinB phosphotransferase gene (hph) and was used to transform A. niger. The high hygromycin resistance transfer-mants thus obtained verified that the synthesized fragment functioned as a promoter in filamentous fungi. Southern blot analysis showed the hph gene has been integrated into genomic DNA of A. niger transformant.