%0 Journal Article
%T Cloning and Function Determination of Promoter Region of Glucoamylase Gene form Aspergillus niger T21<br>黑曲霉糖化酶基因启动子区的克隆及其功能测定
%A Tang Guomin Tang Wenjian Zhong Lichan Yang Kaiyu
%A <br>唐国敏
%A 汤文剑
%A 钟丽婵
%A 杨开宇
%J 生物工程学报
%D 1996
%I 
%X A 850bp fragment of the 5' flanking region of the glucoamylase gene was synthesized from A. niger T21 genome using PCR. The function detection vector was constructed by fusing this fragment to E. colt hygromycinB phosphotransferase gene (hph) and was used to transform A. niger. The high hygromycin resistance transfer-mants thus obtained verified that the synthesized fragment functioned as a promoter in filamentous fungi. Southern blot analysis showed the hph gene has been integrated into genomic DNA of A. niger transformant.
%K PCR
%K glucoamylase
%K promoter<br>PCR
%K 糖化酶
%K 启动子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EF90B7E4FF70CC975012112D029257BA&yid=8A15F8B0AA0E5323&vid=59906B3B2830C2C5&iid=0B39A22176CE99FB&sid=73579BC9CFB2D787&eid=5BC9492E1D772407&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=1